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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude
doi: 10.1084/jem.20202032
Figure Lengend Snippet: Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on CD3ε − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).
Article Snippet: The following antibodies were from
Techniques: Flow Cytometry, Expressing