originpro 8.6 13 software Search Results


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Thermo Fisher biotin-cd3ε (145-2c11, 13-0031-86, 1:400)
Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on <t>CD3ε</t> − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).
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Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on <t>CD3ε</t> − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).
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Illumina Inc human ht-12 beadchips
Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on <t>CD3ε</t> − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).
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Dow Chemical vinyl chloride/vinyl acetate copolymer binder, vmch
Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on <t>CD3ε</t> − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).
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Thermo Fisher biotin-ter-119 antibody
Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on <t>CD3ε</t> − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).
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Image Search Results


Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on CD3ε − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).

Journal: The Journal of Experimental Medicine

Article Title: The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude

doi: 10.1084/jem.20202032

Figure Lengend Snippet: Heterozygous deletion of Tcf7 restores maturation of Id2 Δ/Δ NK cells to the CD27 + CD11b + stage. (A) Flow cytometry showing CD27 and CD11b on CD3ε − NK1.1 + DX5 + NK cells from the BM (top row) or spleen (bottom row) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice ( n = 4 from four independent experiments). (B) Summary of the frequency of each NK cell subset in CD3ε − NK1.1 + DX5 + NK cells from the BM (top) or spleen (bottom) of Ctrl, Id2 Δ/Δ (blue), and Id2 Δ/Δ Tcf7 Δ/+ (green) mice. Error bars are SEM. *, P < 0.05 determined by one-way ANOVA with Tukey’s multiple comparisons test. (C) Intracellular expression of TCF1 or TBET in BM or spleen NK cells from each of the indicated mouse strains. Numbers are MFI. One representative experiment is shown ( n = 2 for Id2 Δ/Δ Tcf7 Δ/+ NK cells and n > 4 for Ctrl, Id2 Δ/Δ , and Id2 Δ/Δ Tcf7 Δ/Δ NK cells).

Article Snippet: The following antibodies were from eBioscience: biotin-TCRβ (H57-597, 13-5961-85, 1:400), biotin-CD3ε (145-2C11, 13-0031-86, 1:400), biotin-γδTCR (UC7-13D5, 13-5811-85, 1:400), PerCp-cyanine5.5-streptavidin (45-4317-82, 1:400), FITC-CD49b (DX5,11-5971-85), PE-CD49b (DX5, 12-5971-82), eFluor450-CD49b (DX5, 48-5971-82), APC-NK1.1 (PK136, 17-5941-82), eFluor450-NK1.1 (PK136, 48-5941-82), PE-cyanine7-CD11b (M1/70, 25-0112-82, 1:400), APC-eFluor780-CD27 (LG.7F9, 47-0271-82), APC-KLRG1 (2F1, 17-5893-82), APC-Ly49D (eBio4E5, 17-5782-82), PE-Ly49H (3D10, 12-5886-82), APC-Ly49E/F (CM4, 17-5848-80), FITC-Ly49G2 (eBio4D11, 11-5781-82), APC-CD226 (10E5, 17-2261-82), and FITC-CD244 (eBio244F4, 11-2441-85, 1:200).

Techniques: Flow Cytometry, Expressing